Intermediate—Easy to perform; requires some background knowledge.
- Designed for 16 to 32 students (8 stations)
- Minimal teacher preparation
- Inquiry-based learning
Teach your students about gene transfer between bacterial strains. In 1 of the included strains of E. coli, the gene for antibiotic resistance for streptomycin is located on the chromosome. In the other, the gene for antibiotic resistance for ampicillin occurs as extrachromosomal plasmid DNA. Students use streaking to determine which bacterial strain is the donor strain. Kit contains perishable materials.
Note: Antibiotic solutions and cultures last 1 month when refrigerated.