- Learn thin layer chromatography technique
- Separate plant pigments using TLC
- Calculate Rf values of separated pigment types
Grades 9–12. Teach your students the fundamentals of thin layer chromatography with this classic experiment in separation science. Students separate plant pigments and identify compounds based on Rf values they calculate. The activities in this lab require approximately 90 min of classroom time, which can be completed on separate days. Materials are sufficient for 1 class of 30 students working in groups of 3.